![]() To detect preferred substrates of the exosome, the iCLIP reads were compared to the transcript abundance using RNA-Seq data. We identified novel circRNAs corresponding to 5′ parts of two homologous, transposase-related mRNAs. Instead, they corresponded to adenine-rich RNA tails, which are post-transcriptionally synthesized by the exosome, and to circular RNAs (circRNAs). A relatively high proportion (17–19%) of the obtained cDNA reads could not be mapped to the genome. To determine binding sites of the exosome to RNA on a global scale, we performed individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) analysis with antibodies directed against aRrp4 and aRrp41 of the chrenarchaeon Sulfolobus solfataricus. Distribution of crosslink sites around the ends of annotated genes Clustering of crosslink sites at the 5′ and 3′ end of predicted operons. Clustering of read counts at the 5′-and 3′-end of protein coding genes. solfataricus was analyzed with respect to biotype RNA. Distribution of read counts in the genome of S. Low affinity of the archaeal exosome to the abundant mRNA tmoA (SSO_RS06040 gene encoding toluene-4-monooxygenase system protein). Binding of the archaeal exosome to 5’and 3’parts of selected genes. Antisense RNAs as preferred substrates of the archaeal exosome. Global analysis of RNAs enriched by coimmunoprecipitation with the archaeal exosome by iCLIP. Poly(A) stretches in RNA are bound by the exosome. Distribution of bases in RNA-tails detected in the aRrp41-iCLIP of the archaeal exosome. CircRNAs identified in the exosome iCLIP analysis of S. The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.Īdditional file 1: Figure S1. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
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